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cloning abemax p2a egfp sequence  (Addgene inc)


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    Addgene inc cloning abemax p2a egfp sequence
    Cloning Abemax P2a Egfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloning abemax p2a egfp sequence/product/Addgene inc
    Average 94 stars, based on 73 article reviews
    cloning abemax p2a egfp sequence - by Bioz Stars, 2026-04
    94/100 stars

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    a, Schematic of GFP ON (GO) base editing reporters utilized to quantify editing activity in B-ALL cells (adapted from Katti et al. 2020 Nucleic Acids Research ; see ref. 85). Base editing enables production of GFP mRNA transcript by de novo creation of start codons (Cy GO ) or removal of premature stop codons (Ad GO ). b, Schematic of experimental strategy for stable expression of base editors in B-ALL cells. Cells either did not recover from antibiotic selection, or had low viability with no detectable reporter activity after enrichment with FACS. c, Flow-cytometry assisted quantification of % GFP positive ABE reporter cells after >10 days of selection for ABE8e-Puro+ cells. Each symbol represents a technical replicate (n=3). Error bars represent mean ± s.d. d, Immunoblot analysis for Cas9 expression in B-ALL cells, or 293T cells transfected with ABE8e Puro construct.

    Journal: bioRxiv

    Article Title: Multiplexed in vivo base editing identifies functional gene-variant-context interactions

    doi: 10.1101/2025.02.23.639770

    Figure Lengend Snippet: a, Schematic of GFP ON (GO) base editing reporters utilized to quantify editing activity in B-ALL cells (adapted from Katti et al. 2020 Nucleic Acids Research ; see ref. 85). Base editing enables production of GFP mRNA transcript by de novo creation of start codons (Cy GO ) or removal of premature stop codons (Ad GO ). b, Schematic of experimental strategy for stable expression of base editors in B-ALL cells. Cells either did not recover from antibiotic selection, or had low viability with no detectable reporter activity after enrichment with FACS. c, Flow-cytometry assisted quantification of % GFP positive ABE reporter cells after >10 days of selection for ABE8e-Puro+ cells. Each symbol represents a technical replicate (n=3). Error bars represent mean ± s.d. d, Immunoblot analysis for Cas9 expression in B-ALL cells, or 293T cells transfected with ABE8e Puro construct.

    Article Snippet: ABE8e (Addgene, cat. no. 138495) was subsequently cloned into this backbone and the Cas9 sequence was replaced with one derived from pLenti-FNLSNG-P2A-Puro (Addgene, cat. No. 136900).

    Techniques: Activity Assay, Expressing, Selection, Flow Cytometry, Western Blot, Transfection, Construct