p2a mcherry caax sequence (Addgene inc)
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p2a mcherry caax sequence
P2a Mcherry Caax Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2a+sequence/pSYC-187+(Plasmid+%2374794)/pmc12546983-269-5-9
Average 86 stars, based on 3 article reviews
P2a Mcherry Caax Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p2a+sequence/pSYC-187+(Plasmid+%2374794)/pmc12546983-269-5-9
Average 86 stars, based on 3 article reviews
p2a mcherry caax sequence - by Bioz Stars,
2026-10
86/100 stars
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Over Expression:Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape. Article Snippet: Tetracycline-induced conditional protein overexpression To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape Article Snippet: To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Sequencing:Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape. Article Snippet: Tetracycline-induced conditional protein overexpression To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells Article Snippet: Four-steps molecular cloning was performed to insert the sequence encoding the mCherry fluorescent protein into pLenti CMV/TO RasV12 Puro (w119-1) (#22262, Addgene, Watertown, NY, USA). .. Firstly, the mCherry sequence, along with the Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. Article Title: An efficient low cost means of biophysical gene transfection in primary cells Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table ) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape Article Snippet: To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: An efficient low cost means of biophysical gene transfection in primary cells. Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table S1) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Article Title: Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells. Article Snippet: Four-steps molecular cloning was performed to insert the sequence encoding the mCherry fluorescent protein into pLenti CMV/TO RasV12 Puro (w119-1) (#22262, Addgene, Watertown, NY, USA). .. Firstly, the mCherry sequence, along with the Article Title: Nucleolar protein TAAP1/ C22orf46 confers pro-survival signaling in non-small cell lung cancer Article Snippet: 1%) in FluoroBrite media containing caspase-3/7 fluorescent Green Dye (4440; Sartorius) followed by incubation for 18 h. Recombinant granzyme B (200 nM, AB285779; Abcam) was mixed with 50 ng/ml streptolysin O (S5265; Merck) and 1 mM DTT in FluoroBrite medium containing caspase-3/7 Green Dye (Sartorius) and incubated for 16 h. The fraction of apoptotic cells was determined by image-based cell counting of all cells (red) and apoptotic cells (green) at a Celigo imaging cytometer (Nexcelom Bioscience). .. For C-terminal tagging of the C22orf46 ORF FLJ23584 (UniProt ID Q9H5C5 ) a homology-directed repair donor plasmid was generated containing an expression cassette encoding eGFP coupled to a blasticidin resistance gene via a Plasmid Preparation:Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape. Article Snippet: Tetracycline-induced conditional protein overexpression To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: An efficient low cost means of biophysical gene transfection in primary cells Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table ) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape Article Snippet: To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: An efficient low cost means of biophysical gene transfection in primary cells. Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table S1) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Article Title: Nucleolar protein TAAP1/ C22orf46 confers pro-survival signaling in non-small cell lung cancer Article Snippet: 1%) in FluoroBrite media containing caspase-3/7 fluorescent Green Dye (4440; Sartorius) followed by incubation for 18 h. Recombinant granzyme B (200 nM, AB285779; Abcam) was mixed with 50 ng/ml streptolysin O (S5265; Merck) and 1 mM DTT in FluoroBrite medium containing caspase-3/7 Green Dye (Sartorius) and incubated for 16 h. The fraction of apoptotic cells was determined by image-based cell counting of all cells (red) and apoptotic cells (green) at a Celigo imaging cytometer (Nexcelom Bioscience). .. For C-terminal tagging of the C22orf46 ORF FLJ23584 (UniProt ID Q9H5C5 ) a homology-directed repair donor plasmid was generated containing an expression cassette encoding eGFP coupled to a blasticidin resistance gene via a Control:Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape. Article Snippet: Tetracycline-induced conditional protein overexpression To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Article Title: Aurora B and INCENP co-overexpression severely disrupts mitosis and distinctly modifies the global transcriptional landscape Article Snippet: To generate cell lines that allowed conditional individual overexpression of each protein, the gene encoding either AurkB or INCENP was cloned downstream of the TET promoter in the pcDNA5 FRT/TO vector (Invitrogen). .. Likewise, to achieve their simultaneous overexpression, both the INCENP and AURKB ORFs, separated by the Amplification:Article Title: Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells Article Snippet: Four-steps molecular cloning was performed to insert the sequence encoding the mCherry fluorescent protein into pLenti CMV/TO RasV12 Puro (w119-1) (#22262, Addgene, Watertown, NY, USA). .. Firstly, the mCherry sequence, along with the Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. Article Title: Oncogene-Induced Senescence Is a Crucial Antitumor Defense Mechanism of Human Endometrial Stromal Cells. Article Snippet: Four-steps molecular cloning was performed to insert the sequence encoding the mCherry fluorescent protein into pLenti CMV/TO RasV12 Puro (w119-1) (#22262, Addgene, Watertown, NY, USA). .. Firstly, the mCherry sequence, along with the Membrane:Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. Polymerase Chain Reaction:Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. Cloning:Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. DNA Ligation:Article Title: Spatial organizations of heterochromatin underpin nuclear structural integrity of ventricular cardiomyocytes against mechanical stress. Article Snippet: In brief Cardiomyocytes exhibit characteristic prominent spatial organizations of heterochromatin (SOH).. Fujiwara et al. found that the replacement of endogenous H2B with H2B-mCherry disrupts SOH, leading to extreme elongation of ventricular cardiomyocyte nuclei, resulting in severe heart failure, demonstrating that SOH play a structural role in maintaining nuclear shape against mechanical stress. Transfection:Article Title: An efficient low cost means of biophysical gene transfection in primary cells Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table ) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Article Title: An efficient low cost means of biophysical gene transfection in primary cells. Article Snippet: .. DNA sequences encoding investigator designed gRNAs (Supplemental Table S1) were inserted into lentiCRISPRV2 plasmid (Addgene #52961) or px459v2 (Addgene # 62988) encoding for guides scaffolds, nuclearly localized Cas9 protein and Puromycin resistance gene linked by Generated:Article Title: Nucleolar protein TAAP1/ C22orf46 confers pro-survival signaling in non-small cell lung cancer Article Snippet: 1%) in FluoroBrite media containing caspase-3/7 fluorescent Green Dye (4440; Sartorius) followed by incubation for 18 h. Recombinant granzyme B (200 nM, AB285779; Abcam) was mixed with 50 ng/ml streptolysin O (S5265; Merck) and 1 mM DTT in FluoroBrite medium containing caspase-3/7 Green Dye (Sartorius) and incubated for 16 h. The fraction of apoptotic cells was determined by image-based cell counting of all cells (red) and apoptotic cells (green) at a Celigo imaging cytometer (Nexcelom Bioscience). .. For C-terminal tagging of the C22orf46 ORF FLJ23584 (UniProt ID Q9H5C5 ) a homology-directed repair donor plasmid was generated containing an expression cassette encoding eGFP coupled to a blasticidin resistance gene via a Expressing:Article Title: Nucleolar protein TAAP1/ C22orf46 confers pro-survival signaling in non-small cell lung cancer Article Snippet: 1%) in FluoroBrite media containing caspase-3/7 fluorescent Green Dye (4440; Sartorius) followed by incubation for 18 h. Recombinant granzyme B (200 nM, AB285779; Abcam) was mixed with 50 ng/ml streptolysin O (S5265; Merck) and 1 mM DTT in FluoroBrite medium containing caspase-3/7 Green Dye (Sartorius) and incubated for 16 h. The fraction of apoptotic cells was determined by image-based cell counting of all cells (red) and apoptotic cells (green) at a Celigo imaging cytometer (Nexcelom Bioscience). .. For C-terminal tagging of the C22orf46 ORF FLJ23584 (UniProt ID Q9H5C5 ) a homology-directed repair donor plasmid was generated containing an expression cassette encoding eGFP coupled to a blasticidin resistance gene via a Clone Assay:Article Title: Nucleolar protein TAAP1/ C22orf46 confers pro-survival signaling in non-small cell lung cancer Article Snippet: 1%) in FluoroBrite media containing caspase-3/7 fluorescent Green Dye (4440; Sartorius) followed by incubation for 18 h. Recombinant granzyme B (200 nM, AB285779; Abcam) was mixed with 50 ng/ml streptolysin O (S5265; Merck) and 1 mM DTT in FluoroBrite medium containing caspase-3/7 Green Dye (Sartorius) and incubated for 16 h. The fraction of apoptotic cells was determined by image-based cell counting of all cells (red) and apoptotic cells (green) at a Celigo imaging cytometer (Nexcelom Bioscience). .. For C-terminal tagging of the C22orf46 ORF FLJ23584 (UniProt ID Q9H5C5 ) a homology-directed repair donor plasmid was generated containing an expression cassette encoding eGFP coupled to a blasticidin resistance gene via a |
